T7體外轉錄試劑盒

Product Name: T7體外轉錄試劑盒

INTRODUCTION: 

The T7-FlashScribe™ Transcription Kit is specially formulated to enable users to obtain the maximum  possible yields of RNA from an in vitro transcription (IVT) reaction in just 30 minutes. The standard  30 minute, 20 μl reaction will yield up to 180 μg of RNA from 1 μg of the control template. These yields  are made possible by the high-performance properties of the T7-FlashScribe enzyme. The T7-FlashScribe Transcription Kit produces exceptionally high yields of either long or short transcripts.  The standard reaction can be scaled up to produce milligram amounts of RNA. T7-FlashScribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through  the use of CELLSCRIPT's ScriptCap™ m7 G Capping System, ScriptCap 2'-O-Methyltransferase Kit and  A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).  CELLSCRIPT also offers the INCOGNITO™ line of transcription kits for the production of pseudouridine- & 5-methyl-cytosine-containing (GA C and GA 5mC) IVT RNA. It has been shown that -mRNAs and  5mC-mRNAs are translated into protein at higher levels and induce lower innate immune responses in  human and other mammalian cells that express various RNA sensors compared to corresponding  unmodified mRNAs.